highly significantP <0.05
The addition of erastin (8 μM) treated group showed decreased differences compared to the PEDV infected group ( P <0.05), while the Fer-1 (60 μM) treated group showed highly significant differences compared to the erastin (8 μM) and Fer-1 (60 μM) co-treated groups ( P <0.05), especially the PEDV nucleotide content was highly significantly reduced in the erastin (8 μM) treated group compared to the Fer-1 (60 μM) treated group ( P <0.001) ( Figure 2A ).