highly significantP = 0.00519
In a GLM with a negative binomial transformation of seed number, the signal for MPK12 G53R on fitness was reduced but still highly significant (GLM, MPK12 53R allele fixed-effect estimate = 0.7, P = 0.00519) ( supplementary table S12, Supplementary Material online), indicating the MPK12 53R variant increases fitness independently from FRI 232X under CVI (Espongeiro) conditions.