Construction of CRISPR-Cas9 Vectors Containing pyrG/amdS Marker Gene CRISPR−Cas9 genome editing technology plays an extremely significant role in synthetic biology and metabolic engineering [ 31 ].
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Development of Marker Recycling Systems for Sequential Genetic Manipulation in Marine-Derived Fungi <i>Spiromastix</i> sp. SCSIO F190 and <i>Aspergillus</i> sp. SCSIO SX7S7.
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