not quite significantP = 0.083
Multivariate analyses Nested analyses on the 4 chick level datasets showed a highly significant effect of box on chick enterobacterial load ( N = 44, F 20,21 = 11.4, P < 0.001), chick total bacteria ( N = 44, F 20,21 = 41, P < 0.001) and chick weight ( N = 44, F 20,21 = 7.97, P < 0.001), while box effect on chick fungal load was not quite significant ( N = 44, F 20,21 = 1.86, P = 0.083).