Alterations in immunophenotype and metabolic profile of mononuclear cells during follow up in children with multisystem inflammatory syndrome (MIS-C).

We could observe an oxidative burst after injection with PMA and ionomycin in isolated monocytes ( Figure S10H ), however, there was no significant difference in the oxidative burst between the PA group and HC with a slight trend (not significant - ns) toward higher oxidative burst in the convalescent ( 14 ) MIS-C patients.

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We did, however, observe a weak trend of higher basal ECAR and ATP production from the oxidative phosphorylation (OxPhos) in the convalescent MIS-C group compared to the control group, but the differences were not significant (p > 0.05, Mann-Whitney test) ( Figures S10B, E ).

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