As reported in Figure 6 , at 24 h post-treatment GFAP mRNA levels showed an increasing trend in all treatments but only in presence of IFN-γ or in correspondence of LPS + IFN-γ statistically significant increases were scored compared with non-treated (NT) cells.
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Cytological, molecular, cytogenetic, and physiological characterization of a novel immortalized human enteric glial cell line.
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Notably, flow cytometry analysis of Ki-67, a well-known marker of cell proliferation ( Sun and Kaufman, 2018 ), showed a significant reduction in the Ki-67 positive sub-population in ClK cells compared with the high-grade astrocytoma T98G cell line as well as a decreasing trend according to cell confluence.