As expected, (based on the spectral properties of the filter used, see Materials and Equipment and Supplementary Figure 2 ), the results of our measurements show a highly significant correlation between the iReenCAM-measured fluorescence and HPLC-measured chlorophyll a content in both ftsh2 and porB mutants and corresponding WT backgrounds ( Col-0 and Ler-1 , respectively; Figures 3C, D ).
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iReenCAM: automated imaging system for kinetic analysis of photosynthetic pigment biosynthesis at high spatiotemporal resolution during early deetiolation.
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