When pseudotime was plotted against UMAP component 1, there was an obvious trend for cultures to progress systematically through pseudotime at the early time points (days 0–4), but there was little separation once they reached 4.5, where more mature micromass cultures were clustered.
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The sentences
There was an overall trend from less mature (undifferentiated) cells on day 0 to more mature (differentiated) micromass cultures on day 10 and towards mature articular chondrocytes, indicating a progressively different transcriptome during chondrogenesis.