We genotyped 153 RILs from this population with the PLATZ-A1 marker and confirmed that Rht25c was associated with a highly significant decrease ( P < 0.001) in plant height relative to Rht25f in a factorial ANOVA including the RHT-D1 gene, which was also segregating in this population ( Dataset S14 ).
A factorial ANOVA using the two homeologs as factors showed significant effects in plant height and peduncle length ( P < 0.0001) for PLATZ-A1 , but marginally nonsignificant effects for PLATZ-B1 ( Dataset S4 ).
No significant differences were detected in kernel number per m 2 for the individual or combined platz1 mutants ( Dataset S4B ), suggesting that the reduction in grain size may be the main contributor to the marginally significant reduction in grain yield observed in this experiment.