Effects of dietary crude protein levels in the concentrate supplement after grazing on rumen microbiota and metabolites by using metagenomics and metabolomics in Jersey-yak.

According to the correlation analysis of differential metabolites in the serum and VFA parameters ( Figure 8A ), results showed that Acetate, Propionate, Butyrate, and L-Carnitine, PC (18:1/0:0), 2-amino-4-({2-[(2-carbon-2-hydroxy-1-phenyl ethyl) sulfinyl]-1-(carbo) Metabolites such as 3-Buten-1-amine and Glucosylsphingosine were negatively correlated with N,N-dimethyl-Safingol, 16-Hydroxy hexadecanoic acid, Polyoxyethylene 40 monostearate and PC [16:0/18:2(9Z, 2z)] and other metabolites showed positive correlation, among which Butyrate and L-Carnitine had extremely significant negative correlation ( p < 0.05), Isovalerate and PC (18:1/0:0) and PC [20:5(5Z,8Z,11Z,14Z,17Z)/0:0].

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