With DAPT treatment, we observed a significant increase in the % NEUROD1 + cell population of the MEL1-INS GFP/w cells and an increase trending toward significance in the % NEUROD1 + population of the H1 cells (Figs. 4 A,B,D,E, and S7 D).
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Protocol development to further differentiate and transition stem cell-derived pancreatic progenitors from a monolayer into endocrine cells in suspension culture.
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