Universal CRISPR/Cas12a-associated aptasensor suitable for rapid detection of small proteins with a plate reader.

By increasing the concentration of the aptamer–cDNA dsDNA from 0.5 nM to 5 nM, the fluorescence intensity also increased ( Figure 4C ), and such an increasing trend can also be directly observed from the solid surface of the high-binding plate by confocal microscopy when the dsDNA concentration increased from 0 nM to 100 nM ( Supplementary Figure S10 ).

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