an increasing trendp = 0.0005
The calculated unit size was not different from zero during individual increments 2–4, although with an increasing trend, and attained significance during decrement 5 (~5 pA, p = 0.0005, different from 0, two-tailed one sample t test) and when the data points from decrements 4 and 5, which had similar mean values, were combined ( p = 0.0017).