This finding was validated in the present study, where Treg numbers continued to show an increasing trend in the first 4 d postoperatively and began to show a decrease on postoperative day 5 after resection of stage 2 ALPPS stage liver tumors.
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CD4<sup>+</sup>CD25<sup>+</sup> regulatory T cells decreased future liver remnant after associating liver partition and portal vein ligation for staged hepatectomy.
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