Also, regarding permeabilization, we initially tried a permeabilization with 0.25% TritonTM X-100, which was discarded as it caused a highly significant alteration in scatter parameters and cell loss during the preparation steps prior to flow cytometry (data not shown).
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Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium subspecies paratuberculosis in vitro.
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