Comparison of NET quantification methods based on immunofluorescence microscopy: Hand-counting, semi-automated and automated evaluations.

In summary, the data showed that semi-automated quantification and fully automated quantification via NETQUANT produced mostly different results from hand counting with an overall heterogenous pattern, ranging from highly significant to similar levels. 3 Discussion Missing a standard quantification technique makes comparisons of results between different research groups, which are working on the topic of NET formation, a challenging task.

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