In both 3′ and 5′ data, the number of cells was lower in the cryopreserved samples, and the difference was marginally significant in the 5′ data (before accounting for the testing of multiple hypotheses); in toto , we interpret these observed reductions as reproducible but of small effect.
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A structured evaluation of cryopreservation in generating single-cell transcriptomes from cerebrospinal fluid.
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