Models for prediction of single base primer extension efficiency from position and type of single mismatch in primer-template duplex.

As Table 1 shows, regression model 1 considering only the position effect of MM in primer-template duplexes ( formula 1 ) showed the relatively high coefficient of determination (R 2 = 0.5466) in despite of linear regression model, and regression model 2 (R 2 = 0.6731) considering both the position effect of MMs and annealing temperature ( formula 2 ) showed the highly significant R 2 , compared to regression model 1.

also in 132,142 other papers