Reverse Transcription Can Critically Impact the Diagnostic Outcome of <i>BCR::ABL1</i> Quantitative Real-Time RT-PCR.

Cancers (Basel) · 2023 · PMC10417499 · PMID 37568730

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a weak trendno p-value reported

This affects both the numerator and denominator quite equally, and therefore, despite a weak trend towards random priming superiority, the cDNA priming type accounts only for minor changes in the diagnostic outcome, which are within the acceptable range (up to threefold) of variability [ 26 ] ( Figure 4 C).

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