The only remarkable finding of these RT-qPCR results is that Prmt4/Carm1 loss reduces expression of Runx2 mRNA (at day 6, p < 0.001), with a numerical trend of decreased expression for Runx2 , Osterix/Sp7 and Dlx5 during the differentiation time-course (Fig.
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Protein arginine methyltransferases PRMT1, PRMT4/CARM1 and PRMT5 have distinct functions in control of osteoblast differentiation.
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However, this numerical trend was not matched by a statistical trend based on standard t -tests compared to controls.