Breeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum).

Changes in the percentage of total ATI from 1891 to 2010 The crude protein content was almost constant for 2017 and showed a decreasing trend from old to modern cultivars for 2015 29 .

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For the average of the three years, an increasing trend was observed between 1891 and 1960, followed by a subsequent decreasing trend until 2010 (Fig. 3b ).

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