an increasing trendp > 0.05
After treatment with additives for 24 h, an increasing trend ( p > 0.05) in the percentage of resistances was observed, with figures of 43.62% (samples treated with SN), 44.17% (Ni) and 47.47% (LA) for the samples of Spanish origin, and percentages of 40.27% (SN), 40.53% (Ni) and 38.40% (LA) for those from Portugal, as shown in Figure 1 .