Assessing the Role of Trypsin in Quantitative Plasma and Single-Cell Proteomics toward Clinical Application.

This comparison of eight trypsins suggested that the peptide and protein numbers between the different proteases show only slight variation. However, an overall trend suggested higher proteome coverage at a 1:50 E:P ratio, which went hand in hand with an increasing number of missed cleaved peptides due to protease concentration ( Supporting Figure 1 ).

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