There is therefore an increasing trend to use extraction methods that do not involve a drying down step, such as the method that combines methanol, water and chloroform in a 3:1:1 ratio in smaller volumes [ 10 ], so direct LC-MS analysis, without drying down, can be performed.
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In Search of Complementary Extraction Methods for Comprehensive Coverage of the <i>Escherichia coli</i> Metabolome.
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