Gene Expression Analysis of Different Organs and Identification of AP2 Transcription Factors in Flax (<i>Linum usitatissimum</i> L.).

In this analysis, the significance levels were set at p < 0.05 to indicate statistically significant differences between treatments and p < 0.01 to indicate highly significant differences. 4.5. qRT-PCR Validation To verify the RNA-seq data’s reliability and quantify the expression of an AP2-ERF transcription factor in flax under saline stress, the SYBR Green I PCR master mix kit (TaKaRa, Maebashi, Japan) was used in the qRT-PCR reactions as in our previous reports [ 70 ].

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