highly significantp < 0.0001
In addition, we found that administration of 3-mercaptopicolinic acid (3-MPA), a known inhibitor of PEPCK ( 13 ), significantly increases the proportion of flux through glycerol GNG relative to PEPCK such that the EM2/EM-calculated f(PEPCK-GNG) = 0.10 ± 0.037 (statistically highly significant, with p < 0.0001, Fig. 3 A ).