In this context, a notable trend in the cryo-EM SPA field in recent years is the development of several different pieces of software to extract protein dynamics and continuous conformational flexibility data from the cryo-EM image datasets, such as 3DVA [ 94 ], MSPACE [ 95 ], e2gmm [ 96 ], Scipion-EM-ProDy workflow [ 97 ] and others.
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Overview of Membrane Protein Sample Preparation for Single-Particle Cryo-Electron Microscopy Analysis.
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