Targeted editing of multiple homologues of GTR1 and GTR2 genes provides the ideal low-seed, high-leaf glucosinolate oilseed mustard with uncompromised defence and yield.

We observed an interesting trend wherein glucosinolate concentration in the margin of GTR ‐edited lines having in‐frame mutations in at least one BjuGTR1 homologue (L18‐16, L34‐3, and L34‐4; hereafter referred to as Category‐I (Ct‐I) lines) ranged from 190.22 to 226.54 μmoles/g DW, which was somewhat higher than the lines having mutations in all the four BjuGTR1 homologues (L4‐11, L4‐15, L15‐19, L30‐13, and L40‐9; referred to as Ct‐II lines) with glucosinolates ranging from 160.10 to 190.95 μmoles/g DW (Figure 5a ).

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