Ca²⁺ addition facilitates the shell repair with eggs production of Pomacea canaliculata through biomineralization and food intaking regulation.

Repeated-measures ANOVA revealed a highly significant change in egg diameter due to shell breakage ( F 1,16 = 164.574, P < 0.001), indicating that the snails with damaged shells produced larger eggs in diameter than those with unbroken shells.

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Shell damage treatments likely influenced the integrity and structure of snail shells, resulting in reduced shell strength, but the shell-broken snail group with calcium carbonate addition showed an increasing trend in shell strength, relative to the shell-broken snail group without calcium carbonate addition, which may also indicate the contribution of calcium carbonate addition.

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canaliculata in different treatments A partially significant variation in snail shell strength was observed among the female groups (Fig. 4 ).

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