Amphipathic Cell-Penetrating Peptide-Aided Delivery of Cas9 RNP for In Vitro Gene Editing and Correction.

Although the gene correction % was stable in cells treated with nanocomplexes containing RNP (20 nM)/HDR template (20 nM) to RNP (40 nM)/HDR template (40 nM) at a 1:1 ratio, increased ratios of 1:2 to 1:4 RNP/HDR template caused a decreasing trend of HDR ( Figure S5A ).

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