a nonsignificant trendP = 0.982
Therefore, we tested three concentrations of LPS (10, 100, and 1,000 ng/mL) and we observed a nonsignificant trend of reduction in organoid size upon 10 ng/mL LPS treatment compared to untreated organoids ( P = 0.982), whereas 100 and 1,000 ng/mL significantly reduced organoid size compared to untreated controls ( P = 0.0027 and 0.0002, respectively) ( SI Appendix , Fig.