When we examined RANBP2-silenced mitotic cells fixed with PFA, we found a highly significant decrease in the overall NuSAP1 signal intensity, which was counteracted by adding MG132, consistent with the Western data ( Figure 2 C).
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Distinct Mitotic Functions of Nucleolar and Spindle-Associated Protein 1 (NuSAP1) Are Controlled by Two Consensus SUMOylation Sites.
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