The luminal intermediate cell proportion (yellow) displays a decreasing trend from E18.5 stage to adult stage. > par ( mar = c ( 5 , 7 , 1 , 7 ), xpd = TRUE ) > barplot ( t (tab_ratio), col =my_colors_15, xlab = "Cell proportion (%)" , + horiz = TRUE , las = 1 ) > legend ( "right" , inset = c (- 0.3 , 0 ), legend = 0 : 5 , pch = 15 , + col =my_colors_15, title = "Cluster" ) Trajectory analysis with monocle3 Constructing trajectories and pseudotime Many biological processes manifest as a dynamic sequence of alterations in the cellular state, which can be estimated through a “trajectory” analysis.
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Unraveling the timeline of gene expression: A pseudotemporal trajectory analysis of single-cell RNA sequencing data.
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