Trade-offs constrain adaptive pathways to the type VI secretion system survival.

Interestingly, while the yjeP I724T mutant was unaffected by changes in osmolarity ( Figures 3 A and 3B), it did exhibit highly significant decreases in maximum growth rate in the exponential phase when grown in alkaline conditions ( Figures 3 C and 3D), suggesting that YjeP may be an ion channel (OD 600 was log-transformed prior to analysis, and the growth rates were assessed with linear regression comparison of the slopes in the exponential phase with significance as detailed in the legend of Figure 3 ).

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