Uncovering the transcriptional responses of tobacco (Nicotiana tabacum L.) roots to Ralstonia solanacearum infection: a comparative study of resistant and susceptible cultivars.

The correlation analysis revealed a highly significant positive correlation between RT-qPCR and RNA-seq data at R-6 h ( r = 0.95 and P = 8.5 × 10 –5 ) and S-6 h ( r = 0.96 and P = 9.8 × 10 –5 ), indicating methodological reliability and supports the consistency of the RNA-sequencing data (Fig. 8 A-H).

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