2P-FLIM unveils time-dependent metabolic shifts during osteogenic differentiation with a key role of lactate to fuel osteogenesis via glutaminolysis identified.

For the remaining time points, there was a decreasing trend of glutamine consumption for both cell culture media conditions (0.01-fold for Osteo + and 0.32-fold for Xpan) (Fig. 2 C).

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On the other hand, BPTES treatment showed an increasing trend of glucose consumption until day 5, followed by a decrease in glucose consumption until day 11 (Fig. 4 D).

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