extremely significantP < 0.0001
(6) Y = 5.89 - 0.73 × X 1 - 0.023 × X 2 - 0.073 × X 3 + 0.13 × X 4 + 0.28 × X 1 X 2 + 0.57 × X 1 X 3 + 0.24 × X 1 X 4 - 0.035 × X 2 X 3 + 0.27 × X 2 X 4 + 0.073 × X 3 X 4 - 1.11 × X 1 2 - 1.29 × X 2 2 - 1.39 × X 3 2 - 0.97 × X 4 2 As shown in Table 3 , the Model was extremely significant ( P < 0.0001), but there was insignificant difference in the Lack of Fit ( P > 0.05), implying that this fitted quadratic polynomial model is accurate and reliable to predict the yield of PWZMLs extraction [35] .