Production of conjugated linoleic acid by lactic acid bacteria; important factors and optimum conditions.

In addition, data showed that with increasing incubation time up to 48 h, CLA production increased and then, a decreasing trend.

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Similarly, at pH = 6 and 7, an increasing trend in CLA biosynthesis was observed for the first 48 h; the isomers produced at pH = 6 and 7 were included, ( c 9, t 11: 31.97 and t 10, c 12: 5.94 and t9, t 11, 9.99 g/mL) and ( c 9, t 11: 24.21 and t 10, c 12: 4.04 and t 9, t 11: 6.31 g/mL), respectively ( Dahiya & Puniya, 2018 ).

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