Simple Chemical and Cholinesterase Methods for the Detection of Nerve Agents Using Optical Evaluation.

Enzyme, Thio-Substrate, and Redox Indicator A highly significant modification of the cholinesterase method was the replacement of the specific substrate acetylcholine with the synthetic substrate acetylthiocholine (later also its analogue butyrylthiocholine), which breaks down to create thiocholine, which reduces 5,5′-dithiobis-2-nitrobenzoic acid (DTNB) to a yellow-coloured product [ 45 ].

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