Relation of Plasma Catecholamine Concentrations and Myocardial Mitochondrial Respiratory Activity in Anesthetized and Mechanically Ventilated, Cardiovascular Healthy Swine.

The inverse non-linear relation of OxPhos plotted as a function of the adrenaline levels narrowly missed statistical significance (r = −0.57, p = 0.051).

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Immunohistochemistry was chosen, because (i) it is well established in the literature that densitometric analysis of colorimetric immunohistochemical staining is as acceptable a method as Western blotting for protein measurement [ 66 ], (ii) we had previously established the immunohistochemistry protocols for porcine cardiac tissue specimens [ 67 , 68 ], (iii) we had obtained highly significant correlations between the densitometric values and those obtained by Western blotting [ 69 ], and (iv) in contrast to Western blotting, the immunohistochemistry evaluation of the tissue allows identification of the physical topography and protein expression in different cell types within the tissue specimen.

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