Most of the DMRs on the Z chromosome were hypermethylated in males, and there were two Z chromosome regions in E6 and E10, with extremely significant differential methylation between males and females, located at Z:27.14Mb-27.39Mb and Z:73.372Mb-73.376Mb, respectively.
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WGBS of embryonic gonads revealed that long non-coding RNAs in the MHM region might be involved in cell autonomous sex identity and female gonadal development in chickens.
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