Simple Western analysis was performed to confirm PLK1 overexpression in melanoma cells and its effect on EMT markers, which showed an increasing trend in the expression of mesenchymal markers, such as N-cadherin and Vimentin, as well as transcription factors ZEB1 and SNAIL (Fig. 3d ).
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Role of PLK1/NUMB/NOTCH in epithelial-mesenchymal transition in human melanoma.
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