Integrated Microbiome and Metabolomics Analysis of the Effects of Dietary Supplementation with Corn-Steep-Liquor-Derived <i>Candida utilis</i> Feed on Black Pigs.

A regression analysis was performed on the data in Table 3 using Design-Expert 12.0 software to obtain a quadratic regression equation for determining the OD values and number of yeast cells in response to the three factors: OD 600 = 0.6760 + 0.0136A + 0.0144B − 0.0025C + 0.0062AB − 0.0140AC − 0.0090BC − 0.0396A 2 − 0.0196B 2 + 0.0006C 2 Number of yeast cells = 2.77 + 0.0800A + 0.0475B + 0.0675C − 0.0150AB − 0.1000AC − 0.0050BC − 0.2930A 2 − 0.0980B 2 − 0.1280C 2 In Table 4 , the F -value was 40.02 and the p -value was < 0.0001, indicating that the model difference was highly significant, and the F-value in the out-of-fit term was 0.9886 and the p -value was = 0.9946 > (0.05), indicating that the difference was not significant enough for an optimal analysis.

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In Figure 1 C, it can be seen that, when the concentration of the glucose master liquor was 8% and the concentration of KH 2 PO 4 was 0.2%, with the gradual increase in the corn steep liquor concentration, there was no significant change in the OD value; additionally, the number of yeast cells showed a decreasing trend in the fermentation liquid of Candida utilis protein feed.

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