highly significantp -value = 0.0099
The difference in Delta Ct (Ct GOI -Ct REF , in which Ct is cycle threshold, GOI is gene of interest, REF is reference gene) median values between PRODH protein-positive and PRODH-negative cases was highly significant ( p -value = 0.0099, ANOVA test), suggesting concordance between protein and transcript levels ( Figure 1 B).