miRNA profiling of chicken follicles during follicular development.

Cluster 1, characterized by a decreasing trend, consisted of the highest number of miRNAs (32 miRNAs) that exhibited high expression in the pre-hierarchical follicles and decreased during the transition from F5 to F1, including POF.

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Moreover, the target genes of Cluster 3, which showed an increasing trend in expression, were associated with the MAPK signaling pathway (Fig. 4 b and Table S5 ).

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