Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes.

We also found a highly significant difference ( p < 0.001 to p < 0.0001; two-tailed Kolmogorov-Smirnov [K-S] test) between the mean radial CT positions of the five largest chromosomes (HSAs 1–5) and those of smaller chromosomes (HSAs 11–22 and Y).

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The mean 3D CT–CN–CT angle obtained for the five largest chromosomes was not significantly different from the mean angle for the small metacentric and submetacentric chromosomes, while a modestly significant difference ( p < 0.05) was noted in mean angle between the latter and the acrocentric chromosomes ( Table 1 ).

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