The effects of NDM-5 on <i>Escherichia coli</i> and the screening of interacting proteins.

The statistical significance was analyzed by the unpaired Student’s t -test and one-way ANOVA (*: p < 0.05; **: p < 0.01; ***: p < 0.001); differences with p < 0.05 were considered significant, those with p < 0.01 were considered very significant, and those with p < 0.001 were considered extremely significant. 3 Results 3.1 Successful construction of E. coli BL21 (pET-28a- bla NDM–5 ) Due to the complex genetic background of clinically isolated bla NDM–5 -positive E. coli , the plasmid pET-28a containing the bla NDM–5 fragment was transformed into wild-type E. coli BL21 to exclude potential interference.

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