Changes of the volatile compounds and odors in one-stage and three-stage infant formulas during their secondary shelf-life.

The peak area ratios of the methyl isobutyl ketone, 2,5-octanedione, α-thujene, and (R)-α-pinene in one-stage samples showed a decreasing trend primarily followed by an increasing trend at 0 day, 3 days and 7 days of simulated daily use.

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The peak area ratios of β-pinene, β-ocimene, and γ-terpinene showed a decreasing trend overall as the number of days after opening increased in one-stage and three-stage samples, probably due to the escape of terpenoids associated with “freshness” into the environment.

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