Dermal FOXO3 activity in response to Wnt/β-catenin signaling is required for feather follicle development of goose embryos (Anser cygnoides).

To test the transfection efficiency of these cells, mRNA expression level results were found difference highly significant of fibroblasts after transfection at 72 h compared with NC group, suggesting that the effect of overexpression and silencing of foxo3 is achieved after transfection ( Figure 5B ).

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