Although the increase in a-smooth muscle actin ( Acta2 ) in db/m mice from 26-week-old to 35-week-old may be due to the effect of aging as well, an increasing trend in Acta2 was observed in 35-week-old db/db mice ( Supplementary Fig. 1 ).
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Renal transcriptome analysis of uninephrectomized db/db mice identified a mechanism for the transition to severe diabetic nephropathy.
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